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cat mab0061  (R&D Systems)


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    Structured Review

    R&D Systems cat mab0061
    Cat Mab0061, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+igg2b+isotype+control/Rat+IgG2B+Isotype+Control/pm41999979-580-11-9
    Average 93 stars, based on 153 article reviews
    cat mab0061 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Injection:

    Article Title: Cutting Edge: CCR9 Promotes CD8 + T Cell Recruitment to the Brain during Congenital Cytomegalovirus Infection.
    Article Snippet: .. To inhibit circulating CCL25, we injected mice i.p. with a neutralizing anti-CCL25 blocking Ab or rat IgG2B Isotype Control (5 mg/mouse; R&D Systems) at days 1, 4, 7, 10, and 13 postinfection. .. mAbs for mouse CD4 (GK1.5), CD8a (53-6.7) CCR9/CD199 (CW1.2), CCR6/CD196 (29-2L17), CXCR7 (8F11-M16), CXCR3/ CD183 (CXCR3-173), CD103 (M290), Thy1.1/CD90.1 (OX-7), TCRb (H57-597), Perforin (S16009A) CCR5/CD195 (HM-CCR5 [7A4]), CXCR4/CD184 (2B11), a4b7/LPAM-1 (DATK32), CD45.1 (A20), CD45.2 (104), TNF-a (MP6-XT22), IFN-g (XMG1.2), and Granzyme B (GB11) were purchased from BD Biosciences, BioLegend, or ThermoFisher Scientific.

    Blocking Assay:

    Article Title: Cutting Edge: CCR9 Promotes CD8 + T Cell Recruitment to the Brain during Congenital Cytomegalovirus Infection.
    Article Snippet: .. To inhibit circulating CCL25, we injected mice i.p. with a neutralizing anti-CCL25 blocking Ab or rat IgG2B Isotype Control (5 mg/mouse; R&D Systems) at days 1, 4, 7, 10, and 13 postinfection. .. mAbs for mouse CD4 (GK1.5), CD8a (53-6.7) CCR9/CD199 (CW1.2), CCR6/CD196 (29-2L17), CXCR7 (8F11-M16), CXCR3/ CD183 (CXCR3-173), CD103 (M290), Thy1.1/CD90.1 (OX-7), TCRb (H57-597), Perforin (S16009A) CCR5/CD195 (HM-CCR5 [7A4]), CXCR4/CD184 (2B11), a4b7/LPAM-1 (DATK32), CD45.1 (A20), CD45.2 (104), TNF-a (MP6-XT22), IFN-g (XMG1.2), and Granzyme B (GB11) were purchased from BD Biosciences, BioLegend, or ThermoFisher Scientific.

    Control:

    Article Title: Cutting Edge: CCR9 Promotes CD8 + T Cell Recruitment to the Brain during Congenital Cytomegalovirus Infection.
    Article Snippet: .. To inhibit circulating CCL25, we injected mice i.p. with a neutralizing anti-CCL25 blocking Ab or rat IgG2B Isotype Control (5 mg/mouse; R&D Systems) at days 1, 4, 7, 10, and 13 postinfection. .. mAbs for mouse CD4 (GK1.5), CD8a (53-6.7) CCR9/CD199 (CW1.2), CCR6/CD196 (29-2L17), CXCR7 (8F11-M16), CXCR3/ CD183 (CXCR3-173), CD103 (M290), Thy1.1/CD90.1 (OX-7), TCRb (H57-597), Perforin (S16009A) CCR5/CD195 (HM-CCR5 [7A4]), CXCR4/CD184 (2B11), a4b7/LPAM-1 (DATK32), CD45.1 (A20), CD45.2 (104), TNF-a (MP6-XT22), IFN-g (XMG1.2), and Granzyme B (GB11) were purchased from BD Biosciences, BioLegend, or ThermoFisher Scientific.

    Article Title: IgG-binding peptide, and specific modification of antibody with said peptide
    Article Snippet: (Materials and Methods) Antibodies Trasutumab (Human IgG1) was purchased from Chugai Pharmaceutical Co., Ltd., and Human IgG2 kappa Isotype Control, Human IgG3 kappa Isotype Control, Human IgG4 kappa Isotype Control, Mouse IgG1 kappa Isotype Control, Mouse IgG2b kappa Isotype Control and Mouse IgG3 kappa Isotype Control were purchased from Crown Bioscience Inc. .. Moreover, Rabbit IgG Isotype Control was obtained from Thermo fisher, Rat IgG1 Isotype Control and Rat IgG2b Isotype Control from R&D Systems, Inc., and Rat IgG2c Isotype Control Antibody from LifeSpan BioSciences. ..

    Article Title: Anti-ICOS antibodies
    Article Snippet: In the case of mouse ICOS binding assay the reference antibody was diluted in BCT media (Gibco #A14351-01) to 120 nM and 5 μL added to plate. .. A rat IgG2b isotype control (R&D systems) was added to negative control wells (R&D Systems) diluted in BCT media to 120 nM and 5 μL added to plate. .. Binding of secreted antibodies to human ICOS was detected by addition of 10 μL of goat anti-mouse IgG (Southern Biotech) directly labelled with Europium cryptate (Cisbio) diluted 1/2000 in HTRF assay buffer.

    Article Title: Antibodies to ICOS
    Article Snippet: In the case of mouse ICOS binding assay the reference antibody was diluted in BCT media (Gibco #A14351-01) to 120 nM and 5 μL added to plate. .. A rat IgG2b isotype control (R&D systems) was added to negative control wells (R&D Systems) diluted in BCT media to 120 nM and 5 μL added to plate. .. Binding of secreted antibodies to human ICOS was detected by addition of 10 μL of goat anti-mouse IgG (Southern Biotech) directly labelled with Europium cryptate (Cisbio) diluted 1/2000 in HTRF assay buffer.

    Article Title: FRA1 controls acinar cell plasticity during murine Kras G12D -induced pancreatic acinar to ductal metaplasia.
    Article Snippet: Article FRA1 controls acinar cell plasticity during murine KrasG12D-induced pancreatic acinar to ductal metaplasia

    Article Title: Alveolar type 2 cell LRP1 is needed for surfactant phospholipid metabolism and pulmonary function in mice.
    Article Snippet: .. For negative controls, the primary antibody was replaced with rat IgG2B isotype control (R&D Systems, MN) at the same concentration as the primary antibody. ..

    Article Title: Biomimetic lipid nanoparticles for RNA delivery to breast cancer microenvironment cells by enhanced homotypic and heterotypic adhesion.
    Article Snippet: • Biomimetic lipid nanoparticles camouflaged with cancer cell membrane nanoghosts were developed for targeted

    Negative Control:

    Article Title: Anti-ICOS antibodies
    Article Snippet: In the case of mouse ICOS binding assay the reference antibody was diluted in BCT media (Gibco #A14351-01) to 120 nM and 5 μL added to plate. .. A rat IgG2b isotype control (R&D systems) was added to negative control wells (R&D Systems) diluted in BCT media to 120 nM and 5 μL added to plate. .. Binding of secreted antibodies to human ICOS was detected by addition of 10 μL of goat anti-mouse IgG (Southern Biotech) directly labelled with Europium cryptate (Cisbio) diluted 1/2000 in HTRF assay buffer.

    Article Title: Antibodies to ICOS
    Article Snippet: In the case of mouse ICOS binding assay the reference antibody was diluted in BCT media (Gibco #A14351-01) to 120 nM and 5 μL added to plate. .. A rat IgG2b isotype control (R&D systems) was added to negative control wells (R&D Systems) diluted in BCT media to 120 nM and 5 μL added to plate. .. Binding of secreted antibodies to human ICOS was detected by addition of 10 μL of goat anti-mouse IgG (Southern Biotech) directly labelled with Europium cryptate (Cisbio) diluted 1/2000 in HTRF assay buffer.

    Sterility:

    Article Title: FRA1 controls acinar cell plasticity during murine Kras G12D -induced pancreatic acinar to ductal metaplasia.
    Article Snippet: Article FRA1 controls acinar cell plasticity during murine KrasG12D-induced pancreatic acinar to ductal metaplasia

    Recombinant:

    Article Title: FRA1 controls acinar cell plasticity during murine Kras G12D -induced pancreatic acinar to ductal metaplasia.
    Article Snippet: Article FRA1 controls acinar cell plasticity during murine KrasG12D-induced pancreatic acinar to ductal metaplasia

    Concentration Assay:

    Article Title: Alveolar type 2 cell LRP1 is needed for surfactant phospholipid metabolism and pulmonary function in mice.
    Article Snippet: .. For negative controls, the primary antibody was replaced with rat IgG2B isotype control (R&D Systems, MN) at the same concentration as the primary antibody. ..

    other:

    Article Title: PD-L1 negatively regulates antifungal immunity by inhibiting neutrophil release from bone marrow
    Article Snippet: If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary M arch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used in this paper: For Flow cytometry PE Rat Anti-Mouse CD274 BD Biosciences 558091; 1:100 Brilliant Violet 421TM anti-mouse Ly-6G Antibody BioLegend 127628; 1:100 PerCP/Cyanine5.5 anti-mouse/human CD11b Antibody BioLegend 101228; 1:100 PE anti-mouse CD45 Antibody BioLegend 103106; 1:100 BV421 Mouse Anti-Human CD66 BD Biosciences 562741; 1:100 eBioscienceTM Fixable Viability Dye eFluorTM 780 InvitrogenTM 103106; 1:100 For microscope Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) CST 4412S; 1:200 PD-L1/CD274 Monoclonal Antibody (Clone # 2B11D11) Proteintech 66248-1-Ig; 1:200 For western blot Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb CST 3776S; 1:1000 Phospho-Stat3 (Ser727) Antibody CST 9134S; 1:1000 Jak2 (D2E12) XP® Rabbit mAb CST 3230S; 1:1000 Stat3 (D3Z2G) Rabbit mAb CST 12640S; 1:1000 Anti-PD-L1 antibody [EPR20529] Abcam ab213480; 1:1000 Rabbit anti-CD274 Polyclonal Antibody Absin abs136046; 1:1000 β-Actin (8H10D10) Mouse mAb CST 3700S; 1:1000 GAPDH (D16H11) XP® Rabbit mAb CST 5174S; 1:1000 Lamin B1 (D4Q4Z) Rabbit mAb CST 12586S; 1:1000 For Neutralization Human Dectin-1 Neutralizing antibody - Monoclonal Mouse IgG1 (Clone # 22H8) Invivogen mabg-hdect; 1μg/ml Mouse IgG1 isotype control (Clone # T8E5) Invivogen mabg1-ctrlm; 1μg/ml GoInVivoTM Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody (Clone # 10F.9G2) BioLegend 124328; 200μg/mice GoInVivoTM Purified Rat IgG2b, κ Isotype Ctrl Antibody (Clone # RTK4530) BioLegend 400666; 200μg/mice Ultra-LEAFTM Purified anti-human CD274 (B7-H1, PD-L1) Antibody (Clone # 29E.2A3) BioLegend 329715; 10μg/ml Ultra-LEAFTM Purified Mouse IgG2b, κ Isotype Ctrl Antibody (Clone # MPC-11) BioLegend 400347; 10μg/ml Ultra-LEAFTM Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody (Clone # 10F.9G2) BioLegend 124338; 10μg/ml Ultra-LEAFTM Purified Rat IgG2b, κ Isotype Ctrl Antibody (Clone # RTK4530) ioLegend 400671; 10μg/ml Mouse CXCL1/GRO alpha /KC/CINC-1 Antibody (Clone # 48415) R&D Systems MAB453; 0.2μg/ml or 5ng/mice once Rat IgG2A Isotype Control (Clone # 54447) R&D Systems MAB006; 0.2μg/ml or 5ng/mice once Mouse CXCL2/GRO beta /MIP-2/CINC-3 Antibody (Clone # 40605) R&D Systems MAB452; 2μg/ml or 40ng/mice once Rat IgG2B Isotype Control (Clone # 141945) R&D Systems MAB0061; 2μg/ml or 40ng/mice once Validation All antibodies were validated before first usage.



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